CONCLUSIONS CAD/CAM all-ceramic crown with fiberglass posts might be an effective method for restoration of endodontically treated premolars with MOF cavity defect.PURPOSE To determine the role of Ca2+ in proliferation,migration and osteogenic differentiation of human dental follicle cells(hDFCs). METHODS hDFCs were isolated and cultured. The source of hDFCs was detected by immunofluorescence staining. Osteogenesis and adipogenic differentiation of hDFCs was detected by alizarin red staining and oil red O staining, to identify its multi-directional differentiation ability. A series of Ca2+ solutions with different concentrations was prepared, CCK8 assay was used to detect the proliferative abilities at 1, 3, 5, and 7 d；migratory ability of 24 h was detected by Transwell assay. Calcium nodules were detected by semiquantitative analysis of alizarin red staining. mRNA expression of osteogenic differentiation related genes was examined by real-time quantitative polymerase chain reaction (RT-qPCR).Statistical analysis was performed using SPSS 17.0 software package. RESULTS Compared with the control group, 3,4 and 5 mmol/L Ca2+ significantly promoted proliferation of hDFCs at 3, 5 and 7 d (P less then 0.05). 3, 4, 5 and 6 mmol/L Ca2+ significantly promoted the migration of hDFCs at 24 h(P less then 0.01). High concentration of Ca2+ had no significant effect on its proliferation and migration. The results of alizarin red staining showed that when Ca2+ concentration reached 4 mmol/L, formation of mineralized nodules were increased(P less then 0.01), and Ca2+ concentration-dependent. RT-qPCR results showed that Ca2+ up-regulated the expression of RUNX2 and OCN in osteogenic differentiation genes (P less then 0.01). CONCLUSIONS Low Ca2+ concentration is beneficial to proliferation and migration, and high Ca2+ concentration is beneficial to osteogenic differentiation of human dental follicle cells.PURPOSE To investigate the effect of LncTUG1 on NK cell killing sensitivity in oral squamous cell carcinoma cells. METHODS Oral squamous cell carcinoma cells were used as experimental objects in vitro. TUG1 siRNA was transfected,the expression of TUG1, cell proliferation, cell apoptosis and NK cell killing rate were detected by qRT-PCR, MTT, flow cytometry and LDH. Bioinformatics software was used to predict that TUG1 and miR-212-3p will target and complement each other, so luciferase reporter vector was constructed and the targeting relationship was identified. TUG1 siRNA and miR-212-3p inhibitor were co-transfected into oral squamous cell carcinoma cells, the effects of miR-212-3p inhibitor on TUG1 siRNA on proliferation, apoptosis and NK cell killing sensitivity of oral squamous cell carcinoma cells were evaluated. The data were analyzed with SPSS 21.0 software package. RESULTS TUG1 siRNA could significantly reduce the expression of TUG1 in oral squamous cell carcinoma cells (P=0.000), decrease cell proliferation (P=0.001), promote cell apoptosis (P=0.000), increase the killing rate of NK cells (P less then 0.01). TUG1 siRNA targeted to increase the expression of miR-212-3p. miR-212-3p inhibitor could reverse the effects of TUG1 siRNA on proliferation, apoptosis and NK cell killing rate of oral squamous cell carcinoma cells. CONCLUSIONS Down-regulation of TUG1 targeting and negative regulation of miR-212-3p inhibits proliferation, induces apoptosis and improves NK cell killing sensitivity of oral squamous cell carcinoma cells.PURPOSE To investigate the effect of osthole on periodontal remodeling during orthodontic tooth movement (OTM) in rats. METHODS Seventy two 8-week-old male SD rats were randomly equally divided into 3 groups two experimental groups of osthole with low (20 mg/kg) and high (40 mg/kg) concentration and the control group. Models of OTM were routinely established. Rats in the experimental groups were respectively given osthole by intragastric administration, while rats in the control group received the same volume of solvent. The rats were sacrificed on 7th, 14th, 21st and 28th day after orthodontic treatment, and the maxilla was harvested and the distance between the first and second molar was measured in each stage. Hematoxylin-eosin (H-E) staining, tartrate resistant acid phosphatase (TRACP) staining were performed. The results were analyzed with SPSS 20.0 software package for one-way analysis of variance. https://www.selleckchem.com/products/gm6001.html RESULTS The mesio-moving distance of the three groups successively increased gradually. On the 7th day, there was no difference between the low concentration group and the control group (P&gt;0.05); at other time point, the experimental groups exhibited significant differences from the control group(P0.05). CONCLUSIONS Osthole could increase the number of osteoclast in periodontium and promote bone remodeling at the early stage of treatment, its effect is dose-dependence during OTM.OBJECTIVES Previous literature has reported that physical frailty (PF) closely associated with cognitive impairment (CI). In this study, we aim to describe and evaluate the prevalence and associated factors with different patterns of PF and cognitive impairment. DESIGN A cross-sectional study. SETTING AND PARTICIPANTS Community-dwelling older adults aged ?60 from the West China Health and Aging Trend (WCHAT) study were included in this study. MEASUREMENTS Participants were assessed the PF and cognitive impairment. PF was assessed using the physical phenotype as defined by Fried's criteria. Cognitive impairment was identified using the Short Portable Mental Status Questionnaire (SPMSQ). According different patterns of PF and cognitive impairment, participants were divided into 4 groups not-PF and cognitive intact ( "neither" group), not-PF and cognitive impairment ("CI only" group), PF and cognitive intact ("PF only" group), and PF and cognitive impairment ("both" group). Multinomial logistic regression was uspredict the risk of "PF only", "CI only" and "both".OBJECTIVES Data regarding the occurrence of risk factors that promote the loss of muscle mass, strength and function in sarcopenia patients in elderly Chinese patients are sparse. Here, we investigated the alterations in muscle mass, function and strength in those with sarcopenia over a 4-year period. We further evaluated the risk factors leading to a loss of grip strength, gait speed and skeletal muscle mass index in sarcopenia patients. DESIGN A face-to-face cross-sectional survey. SETTING AND PARTICIPANTS The study population consisted of 560 Chinese aged over 59 years. MEASUREMENTS Study recordings took places over a four-year period from 2014. Muscle mass was assessed through bioelectrical impedance analysis (BIA) performed on an Inbody720, Biospace. Hand-grip strength and 6 m walking speed were used as measurements of muscle strength and function. Sarcopenia was diagnosed using the Asian Working Group for Sarcopenia criteria (AWGS). RESULTS In total, 474 of the elderly residents completed the follow-up.